Part:BBa_K3733034:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3733034

We transform E.coli DH5α strain with BBa_K3733034 and chose neGFP (https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM, 10mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD₆₀₀ in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

Figure 1.Comparison of OD₆₀₀ corrected RFU values under induction of different concentrations of thiosulfate

User Reviews

UNIQb526f30afcc5c671-partinfo-00000001-QINU UNIQb526f30afcc5c671-partinfo-00000002-QINU